Generator
BAT2/THI3

Part:BBa_J45398:Experience

Designed by: André Green II   Group: iGEM06_MIT   (2006-10-28)

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Applications of BBa_J45398

Intended as enzymes generator for catalyzing production of isoamyl alcohol from the cellular metabolite leucine.

User Reviews

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Reshma Shetty

BBa_J45398, when used in the banana odor biosynthetic system BBa_J45900, may not be functional since no isoamyl acetate is detected.

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Characterization

Gas chromatography analysis of banana odor biosynthetic system

The banana odor biosynthetic system (BBa_J45900) includes the BAT2 & THI3 enzyme generator (BBa_J45398) under the control of a constitutive promoter (BBa_R0011).

No isoamyl acetate production from cellular metabolites by banana biosynthetic system
We designed and constructed a system for banana odor production from intracellular metabolites (BBa_J45900). We analyzed cultures with the banana odor biosynthetic system for isoamyl acetate production but did not measure detectable levels of isoamyl acetate by gas chromatography (A). The banana odor generator (BBa_J45200) in the absence of exogenous isoamyl acetate does not produce measurable quantities of isoamyl acetate (B). For reference, we analyzed a sample with 100 ppm pure isoamyl acetate as well (E). Most E. coli strains produce indole. Octyl acetate was used as an internal standard for all samples containing isoamyl acetate.