Generator
BAT2/THI3
Part:BBa_J45398:Experience
Designed by: André Green II Group: iGEM06_MIT (2006-10-28)
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how you used this part and how it worked out.
Applications of BBa_J45398
Intended as enzymes generator for catalyzing production of isoamyl alcohol from the cellular metabolite leucine.
User Reviews
UNIQ1ef4dfee632891ab-partinfo-00000000-QINU
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BBa_J45398, when used in the banana odor biosynthetic system BBa_J45900, may not be functional since no isoamyl acetate is detected. |
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Characterization
Gas chromatography analysis of banana odor biosynthetic system
The banana odor biosynthetic system (BBa_J45900) includes the BAT2 & THI3 enzyme generator (BBa_J45398) under the control of a constitutive promoter (BBa_R0011).
No isoamyl acetate production from cellular metabolites by banana biosynthetic system
We designed and constructed a system for banana odor production from intracellular metabolites (BBa_J45900). We analyzed cultures with the banana odor biosynthetic system for isoamyl acetate production but did not measure detectable levels of isoamyl acetate by gas chromatography (A). The banana odor generator (BBa_J45200) in the absence of exogenous isoamyl acetate does not produce measurable quantities of isoamyl acetate (B). For reference, we analyzed a sample with 100 ppm pure isoamyl acetate as well (E). Most E. coli strains produce indole. Octyl acetate was used as an internal standard for all samples containing isoamyl acetate.
We designed and constructed a system for banana odor production from intracellular metabolites (BBa_J45900). We analyzed cultures with the banana odor biosynthetic system for isoamyl acetate production but did not measure detectable levels of isoamyl acetate by gas chromatography (A). The banana odor generator (BBa_J45200) in the absence of exogenous isoamyl acetate does not produce measurable quantities of isoamyl acetate (B). For reference, we analyzed a sample with 100 ppm pure isoamyl acetate as well (E). Most E. coli strains produce indole. Octyl acetate was used as an internal standard for all samples containing isoamyl acetate.